Primer

Part:BBa_K5492020

Designed by: Norbert Nyeste   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-17)
Revision as of 15:07, 1 October 2024 by Vojtech (Talk | contribs)


Genscript adapter forward primer

Forward primer sequence of the adapter sequence utilised by GenScript. Utilised in PCR reactions for part BBa_K5492402 and BBa_K5492403.

Experiments

PCR – HNMT

We have found the following PCR protocol the most optimal for the part: hnmt-pcr-plan.png

According to the previous PCR reaction we decided to use S1 and N1 (from our previous reactions.) We added the following substrates in order: (each optimized and wild type)

hnmt-pcr-substr.png

We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DNA solution in the following order: 

hnmt-pcr.png 1. S1O (optimized) 2. S1W (wild type) 3. N1O (optimized) 4. N1W (wild type) 5. Ladder


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None