Regulatory

Part:BBa_K174004:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-10)
Revision as of 15:00, 21 October 2009 by Goksel (Talk | contribs) (→‎Source)

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Pspac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pMUTIN2's genbank accession number is AF072806. Although there is no sequence information for pMUTIN4, it can be derived from pmutin2's sequence information by changing the O1 LacI binding site with the "oid" sequence.

Source

This sequence is taken from pMUTIN4 integration vector. pmutin2's LacI binding site was changed with the "oid" sequence to create pMUTIN4.

References

  1. Vagner, V., E. Dervyn, et al. (1998). "A vector for systematic gene inactivation in Bacillus subtilis." Microbiology 144(11): 3097-3104.
  2. Lehming, N. et al. The interaction of the recognition helix of Lac repressor with lac operator. EMBO J. 6, 3145−3153 (1987).