DNA

Part:BBa_K5492731:Design

Designed by: Vojtech Vasut, Sandor Ban, Norbert Nyeste, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 10:42, 19 September 2024 by Noriii (Talk | contribs) (→‎Design Notes)

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J1_aptamer_reverse_complement_strand


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ssDNA sequence designed to be complementary for the 5' end of j1_aptamer_with_fluorophore, it contains a quencher molecule which absorbs the light emitted by fluorophore at its 3' end, until the aptamer binds histamine.

Source

https://www.sciencedirect.com/science/article/abs/pii/S0039914019311075?via%3Dihub Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.

References

Reference: Zhao, Q.; Fan, T.; Chen, L.; Fu, B.; Zhou, Y.; Zheng, X.; Wu, J. Development of Aptamers for Recognition and Separation of Toxins. Talanta 2020, 211, 120725. DOI: 10.1016/j.talanta.2020.120725.