Coding

Part:BBa_K5492202:Design

Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 17:25, 24 September 2024 by Noriii (Talk | contribs) (→‎Source)


Wild type rDAO - pETDUET-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal BglII site found at 1050
    Illegal BglII site found at 2303
    Illegal BamHI site found at 1978
    Illegal BamHI site found at 2232
    Illegal XhoI site found at 848
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites adapted to pT7CFE1, with adjustions also added for proper framing of the gene.


Source

The work of iGEM20_SEHS-China (https://parts.igem.org/Part:BBa_K3684002 BBa_K3684002) provided the dna sequence of rDAO.

References