DNA

Part:BBa_K5036021

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-12)
Revision as of 08:23, 14 September 2024 by Em100 (Talk | contribs) (→‎Part Description)


UAS Trans CMV enhancer

Part Description

it is a a Powerful Tool for Gene Expression which is derived from the cytomegalovirus (CMV). it can significantly boost gene expression in various cell types. it works by binding to specific transcription factors, which then recruit other proteins to the promoter region of a gene. This complex interaction helps to initiate and sustain gene transcription.

Usage

This part is fused with GAL4, dcas9(c), VP64. it acts as a powerful transcription activator where it induce transcription and increased gene expression so after receptor activation, it will be directed with dCas9,VP64 and GAL4 to increases expression of YAP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None