Coding

Part:BBa_K4839024

Designed by: Xuming Zeng   Group: iGEM23_SYSU-SLS-CHINA   (2023-10-03)
Revision as of 15:59, 12 October 2023 by Yulujun (Talk | contribs)

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pGK-GFP-IRF4


This part is design for the validification of our BioPROTC system. In our design, we fused GFP and IRF4 together and thus we can use both anti-GFP or anti-IRF4 BioPROTAC to detect the degradation efficiency of both protein.(Figure 1.)



Figure1. The function of anti-IRF4 BioPROTAC

We would like to construct GFP-IRF4 using visualization methods to simulate the degradation process of IRF4 by anti-IRF4 BioPROTAC. Therefore, we have proposed the following design, and we have also designed alternative options, namely pcDNA3.1-CMV-FLAG-IRF4 and pcDNA3.1-CMV-HA-IRF4. The sequence diagrams of GFP-IRF4 and the alternative options FLAG-IRF4 and HA-IRF4 are shown in Figure 2.

Figure 2. The sequence diagrams of GFP-IRF4 and the alternative options FLAG-IRF4 and HA-IRF4.


Since we were unable to complete the construction of GFP-IRF4 within a short period of time, we have chosen to use pcDNA3.1-CMV-FLAG-IRF4 and pcDNA3.1-CMV-HA-IRF4 as our targets for degrading IRF4. We have successfully achieved expression and conducted the following validations of the functionality of anti-IRF4 BioPROTAC.

we transfected HEK293T cells in a 12-well plate with 1 μg of pLVX-TREG3S-FLAG-PU.1-(SSG)3-SPOP-TetOne-Puro and 1 μg of pcDNA3.1-CMV-FLAG-IRF4/pcDNA3.1-CMV-HA-IRF4, induced expression with 1 μg/mL of doxycycline for 48h, and collected the protein for western blot analysis. The results are shown in Figure 3-2, where we observed significant degradation of both HA-IRF4 and FLAG-IRF4.


Figure 3. Western blot signing degrdation of IRF4. "D" represents doxycycline.

Due to time constraints, we have not been able to conduct this experiment in the THP-1 cell line. However, in the HEK293T cell experiment, we have observed a certain degree of IRF4 degradation, which is a breakthrough that has not been achieved in previous research. We are very excited about these results. We will then continue to optimize the transfection/infection efficiency for THP-1 cells and have also contacted Professor Chong Wu, a macrophage expert from Sun Yat-sen University School of Life Sciences, to explore ways to optimize related experiments for THP-1 cells. Additionally, we will conduct further experimental validation for the designed anti-IRF5 BioPROTAC and optimize anti-IRF4 BioPROTAC using bioinformatics methods to enhance its degradation efficiency.

The overall design of our project

In our design, the engineered macrophages, SYN-MACRO could recognize GPC3 via SNIPR. Through Notch signaling pathway, SNIPR will be cleaved 3 times by γ-secretase and release transcription factor Gal4VP64 to activate AntiIRF4-BioPROTAC expression[19], effectively degrading the key protein IRF4 that regulates macrophage M2 polarization. Thereby promoting macrophage M1 polarization (Figure 4). Further SYN-MACRO will release pro-inflammatory cytokines and recruit CD8 T cells for effective tumor eradication.

Figure 4. GPC3 induced M1 polarization by activation of anti-IRF4 BioPROTAC.

After effective tumor eradication, doxycycline is used to induce downstream AntiIRF5-BioPROTAC expression to restore the anti-inflammatory phenotype in the tumor microenvironment also as a safety module, while inhibiting chronic inflammation caused by triglyceride accumulation in liver tissues of many HCC patients, thereby reducing further damage to liver tissue caused by chronic inflammation, also for inhibition of M1 over-polarization (Figure 5)

Figure 5. Doxycycline induced M1 polarization by activation of anti-IRF5 BioPROTAC.

Figure 6. Schematic of the SYN-MACRO M1/M2 polarization.

So now we have the complete gene circuit design of SYN-MACRO (Figure 6). We can control the polarization process of SYN-MACRO in vivo. We hope to precisely target GPC3-positive liver cancer cells with SYN-MACRO and effectively polarize them towards the M1 phenotype, thereby restructuring the tumor microenvironment, promoting the occurrence of inflammation, and inhibiting the development of tumors.



Reference

[1] Rothbauer, U. et al. Targeting and tracing antigens in live cells with fluorescent nanobodies. Nat Methods 3, 887–889 (2006).

[2] Shin, Y. J. et al. Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins. Sci Rep 5, 14269 (2015).

[3] Shen, H. et al. MDM2-Mediated Ubiquitination of Angiotensin-Converting Enzyme 2 Contributes to the Development of Pulmonary Arterial Hypertension. Circulation 142, 1190–1204 (2020).

[4] Klichinsky, M. et al. Human chimeric antigen receptor macrophages for cancer immunotherapy. Nat Biotechnol 38, 947–953 (2020).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2527
    Illegal SpeI site found at 257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2627
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 257
    Illegal NgoMIV site found at 2052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99
    Illegal BsaI.rc site found at 2358
    Illegal SapI site found at 1510
    Illegal SapI.rc site found at 1441


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