Regulatory

Part:BBa_K4613006

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 14:30, 12 October 2023 by Jiandong (Talk | contribs) (→‎Reference)

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Ptra

TraR protein forms a complex with its signaling molecule. This complex binds to the Ptra promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II、-35 sequences、-10 sequences and an RBS.

Reference

  1. White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
  2. Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None