Part:BBa_K4960023

BBa_K4960023
This part is responsible for payloads loading Cre.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 599
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 285
  
• 1000
  COMPATIBLE WITH RFC[1000]

This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows: 5' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3' 3' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5' HiBiT is a polypeptide tag, can be linked to any protein. This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.