Composite

Part:BBa_K4613023

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)
Revision as of 08:46, 10 October 2023 by ZhenyuLi (Talk | contribs)


pET-46 Ek_LIC-ADH3

The composite part was constructed to achieve solube expression of ADH3 and analyze the function of ADH3. The composite part can be directly imported into plasmid and express ADH3 induced with IPTG.


Reference

  1. Dai L, Niu D, Huang J W, et al. Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J]. Journal of Hazardous Materials, 2023: 131836.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 827
    Illegal AgeI site found at 515
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassis
function