Part:BBa_K4593022:Design

Integrative device of endolysin production-release module

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2672
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 3567
  Illegal XhoI site found at 4510
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 5009
  Illegal AgeI site found at 5147
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

ez

Source

The genes encoding SPN1S endolysin and Rz-like spanin (SPN1S_0028 and SPN1S_0029) originate from Salmonella Typhimurium phage SPN1S (GenBank accession number NC_016761)(Lim et al., 2012). The gene encoding LysDZ25 originates from S. aureus phage DZ25 (Chang et al., 2023).

References

Chang, Y., Li, Q., Zhang, S., Zhang, Q., Liu, Y., Qi, Q., & Lu, X. (2023). Identification and Molecular Modification of Staphylococcus aureus Bacteriophage Lysin LysDZ25. ACS Infectious Diseases, 9(3), 497–506. https://doi.org/10.1021/acsinfecdis.2c00493

Lim, J.-A., Shin, H., Kang, D.-H., & Ryu, S. (2012). Characterization of endolysin from a Salmonella Typhimurium-infecting bacteriophage SPN1S. Research in Microbiology, 163(3), 233–241. https://doi.org/10.1016/j.resmic.2012.01.002