Coding

Part:BBa_K2507000

Designed by: Xiaoxiao Chen   Group: iGEM17_SHSBNU_China   (2017-10-01)
Revision as of 03:25, 3 October 2023 by ASHIELIW (Talk | contribs)

ThsS

Background

ThsS (BBa_K2507000) and ThsR (BBa_K2507001), both codon-optimized for E. coli, are two basic parts which belong to the two-component system from the marine bacterium Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell, and ThsR is the DNA-binding response regulator(RR).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 477
    Illegal BamHI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1010

Reference

Daeffler, K. N., Galley, J. D., Sheth, R. U., Ortiz‐Velez, L. C., Bibb, C. O., & Shroyer, N. F., et al. (2017). Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Molecular Systems Biology, 13(4), 923.

Jackson MR, Melideo SL, Jorns MS (2012) Human sulfide: quinone oxidoreductase catalyzes the first step in hydrogen sulfide metabolism and produces a sulfane sulfur metabolite. Biochemistry 51: 6804 – 6815

Levitt MD, Furne J, Springfield J, Suarez F, DeMaster E (1999) Detoxification of hydrogen sulfide and methanethiol in the cecal mucosa. J Clin Invest 104: 1107 – 1114

Schmidl SR, Sheth RU, Wu A, Tabor JJ (2014) Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol 3: 820 – 831

Vitvitsky V, Yadav PK, Kurthen A, Banerjee R (2015) Sulfide oxidation by a noncanonical pathway in red blood cells generates thiosulfate and polysulfides. J Biol Chem 290: 8310 – 8320




Bacterial thiosulfate sensors

Thiosulfate (S2O32-) is a promising biomarker for gut inflammation. Bacterial sensors based on ThsS/R, two-component systems, can be used to diagnose colonic inflammation (colitis) through flow cytometry analysis of colon and fecal samples by responding to thiosulfate.

In a previous study, the sensitivity and specificity of thiosulfate sensors were measured.

Figure 1:Characterization of the thiosulfate sensor ThsSR. (Kristina N-M Daeffler et al. 2017)

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