Device
Part:BBa_K173010:Experience
Designed by: Lorenzo Pasotti, Susanna Schiavi, Paolo Magni Group: iGEM09_UNIPV-Pavia (2009-10-15)
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how you used this part and how it worked out.
Applications of BBa_K173010
This part was not working in a preliminary induction test.
Materials
- Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
- Antibiotics were: Ampicillin (Amp) 100 ug/ml, Kanamycin (Kan) 50 ug/ml and Chloramphenicol (Cm) 12.5 ug/ml. All of them were stored at -20°C in 1000x stocks. Amp and Kan were dissolved in water, while Cm was dissolved in ethanol 100%.
- LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
- M9 supplemented medium was prepared according to: [http://openwetware.org/wiki/Knight:M9_supplemented_media Openwetware protocol].
- 3OC6-HSL (Sigma) was dissolved in water and stored at -20°C in a 2mM stock.
- aTc (Clontech) was dissolved in ethanol 50% and stored at -20°C in a 100 ug/ml stock. All the following dilutions were performed in water.
- Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.
User Reviews
UNIQ218afcd59e96f252-partinfo-00000000-QINU UNIQ218afcd59e96f252-partinfo-00000001-QINU