Coding
RFC37

Part:BBa_K245115:Design

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-10-14)
Revision as of 18:20, 21 October 2009 by Spela (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

KSI-DP-P2/33


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.


Source

The part was multiplied by PCR from pET31-b vector containing KSI-DP with inserted sequence for antimicrobial peptide P2/33.

References