Composite

Part:BBa_K4225019:Design

Designed by: Janviere Yau   Group: iGEM22_HKUST   (2022-10-09)
Revision as of 13:02, 12 October 2022 by Lrsalim (Talk | contribs) (→‎References)


Ptac-TEV-Pc-GFP-LVA-CS-IS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1020
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 855
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1759
    Illegal SapI site found at 1902
    Illegal SapI.rc site found at 439
    Illegal SapI.rc site found at 787


Design Notes

This composite part is codon optimized for our chassis, DH5α. Spacers are also added between the parts.