Coding

Part:BBa_K4275032:Design

Designed by: Maggie Li   Group: iGEM22_GreatBay_SCIE   (2022-10-10)
Revision as of 01:16, 14 October 2022 by Jerryatcg (Talk | contribs) (→‎Source)

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PfuFerritin-Nb3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 547
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The amino acid sequence of Pfuferritin is derived from the genomic sequence of Pyrococcus furiosus whilst its DNA sequence is codon-optimized based on the codon-usage table of E.coli Strain K12.MG1655.

2. An alpha-helice is omitted at the C temrinus of the original Pfuferritin, to accomodate the newly inserted Nb3 domain.

3. The ferritin domain and the Nb3 domain are interspaced by a flexible GS linker (GGGSGGGGSGGG) to avoid the disruption of the oligomerization of ferritin subunits into a 24-mer.


Source

Pyrococcus furiosus
Lama glama (Nb3)

References

1. Fan, Kelong et al. “Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.” Analytical chemistry vol. 90,9 (2018): 5671-5677. doi:10.1021/acs.analchem.7b05217