Composite

Part:BBa_K4146507

Designed by: Yujie Zhang   Group: iGEM22_BUCT   (2022-09-16)
Revision as of 03:08, 8 October 2022 by ZYJ-BUCT (Talk | contribs)


pCS-aroGfbr

aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts. 

 Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.

The standard curve for determination of 2-phenylethanol yield.png 

          The standard curve for determination of 2-phenylethanol yield


The experimental 2-phenylethanol yield.png 

          The experimental 2-phenylethanol yield

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 450
    Illegal EcoRI site found at 665
    Illegal XbaI site found at 87
    Illegal XbaI site found at 941
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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