Composite

Part:BBa_K4368000:Design

Designed by: Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez   Group: iGEM22_UMA_MALAGA   (2022-09-26)
Revision as of 22:51, 6 October 2022 by Alonso (Talk | contribs)


pcstA + rbs + yebF + cenA + terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 554
    Illegal NotI site found at 1698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
    Illegal BglII site found at 1611
    Illegal BamHI site found at 747
    Illegal XhoI site found at 1109
    Illegal XhoI site found at 1358
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 889
    Illegal NgoMIV site found at 1814
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 793


Design Notes

Consideration should be given to the correct optimization of the sequence for use in different organisms.


Source

The cenA gene is from Cellulomonas fimi, while the remaining parts are from Escherichia coli. The whole construct has been optimised for use in E. coli.

References