Plasmid

Part:BBa_K4251027

Designed by: Shi Lifang   Group: iGEM22_SubCat_Shanghai   (2022-09-21)
Revision as of 08:57, 13 October 2022 by Zhuke18 (Talk | contribs)

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GV248-PDRG1-SiRNA-3

GV248-PDRG1-SiRNA-3

Usage and Biology

GV248-vector is one of the most commonly used siRNA expression vectors, which uses the hU6 promoter to regulate the expression of siRNA. The vector contains an EGFP gene to verify if the plasmid was transfected into the host. We inserted the siRNA3 (BBa_K3251005) which targeted PDRG1, into the AgeI and EcoRI sites and regulated by the hU6 promoter. When expressed in the prokaryotic system, the Amp+ resistance can be used to screen the right colony, when transfected into mammalian cells, the puromycin resistance could be used to screen the transfected cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 4265
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2756
    Illegal BglII site found at 3595
    Illegal BglII site found at 5369
    Illegal BglII site found at 5639
    Illegal BglII site found at 8329
    Illegal BglII site found at 8703
    Illegal BamHI site found at 5347
    Illegal XhoI site found at 10163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 185
    Illegal NgoMIV site found at 246
    Illegal NgoMIV site found at 360
    Illegal NgoMIV site found at 8083
    Illegal NgoMIV site found at 9282
    Illegal AgeI site found at 6812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 805
    Illegal SapI.rc site found at 4229


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