Part:BBa_K3714002
Biosensor gard-337 for lateral flow assay
The template of aptazyme gard-337 with two probes (binding BBa_K3714007 and BBa_K3714008) on both terminal for lateral flow assay (LFA). It could be used as a biosensor of gardiquimod to verify our design on a colloidal gold based detection platform (Figure.1).
The probes was chosen manually before tested with RNA structure prediction model. We minimized the interaction between aptazyme domain and two probes. We also checked the possibility of aptazyme dimer.
Then we tested if the probes added would interrupt the activity of aptazyme. We use same method to determine its cleave fraction under a concentration gradient of gardiquimod (Figure.1). We found that the probes added do have a considerable but bearable influence in the sensibility of aptazyme (Figure.1, also see BBa_K3714000).
Next, we questioned if this influence would damage its potential in LFA. The design of LFA could be checked in.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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