Part:BBa_K3735888
SeNPs production parts
In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model (more details see CO Model).
We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.
Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM.
Second, according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16℃, 30℃, and 37℃, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.
Fig.2 Response surface experiment of SeNPs synthesis.
We used response surface model to simulate the optimal SeNPs production conditions (more details see CO Model)). Finally, we obtained the optimal conditions and maximum yield as follows：
Solutions:
By response surface model analysis, we simulated the optimal experimental conditions of SeNPs production: temperature at 30.27℃, selenite concentration at 14.33mM, pH at 6.75. Finally, the optimal SeNPs yield of 0.0315562g was obtained.
Fig.3 the final product of our project.
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