Help:Plasmid Backbone Availability 2009
The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.
- YES - The plasmid is available and correct
- YES[?] - The plasmid is available and correct, with the exception of one inconsistency in the QC results which should not be an issue
- NO - The plasmid is either unavailable or we have found that it carries an error
| Plasmid | J04450 | P1010 | Alt. Location | Insert (click for QC) |
|---|---|---|---|---|
| pSB1A3 | YES Kit Plate 1, well 1K |
YES Kit Plate 1, well 3K |
Kit Plate 2, well 10G [more] | BBa_I732205 |
| pSB1AC3 | YES [?] Kit Plate 1, well 11A |
YES [?] Kit Plate 1, well 11C |
Kit Plate 2, well 22M [more] | BBa_M1001 |
| pSB1AK3 | NO | NO | Kit Plate 3, well 20A [more] | BBa_K143053 |
| pSB1AT3 | YES Kit Plate 1, well 15A |
YES [?] Kit Plate 1, well 15C |
Kit Plate 3, well 1B [more] | BBa_K115001 |
| pSB1A10 | NO | NO | NO | |
| pSB4A5 | NO | YES Kit Plate 1, well 3M |
NO | |
| pSB6A1 | NO | NO | Kit Plate 2, well 14G [more] | BBa_I739001 |
| pSB1C3 | YES Kit Plate 1, well 5A |
YES Kit Plate 1, well 5E |
NO | |
| pSB3C5 | NO | YES Kit Plate 1, well 5G |
NO | |
| pSB4C5 | NO | YES Kit Plate 1, well 5I |
Kit Plate 3, well 5F [more] | BBa_K098010 |
| pSB1K3 | YES [?] Kit Plate 1, well 7A |
NO | NO | |
| pSB2K3 | NO | NO | Kit Plate 2, well 11B [more] | BBa_E0033 |
| pSB3K3 | NO | NO | Kit Plate 2, well 15L [more] | BBa_I714891 |
| pSB4K5 | YES [?] Kit Plate 1, well 7G |
NO | Kit Plate 2, well 17H [more] | BBa_T9003 |
| pSB1AK8 | YES [?] Kit Plate 1, well 13C |
NO | Kit Plate 2, well 13A [more] | BBa_I712006 |
| pSB1T3 | YES [?] Kit Plate 1, well 9A |
NO | NO | |
| pSB3T5 | YES [?] Kit Plate 1, well 9C |
YES [?] Kit Plate 1, well 9G |
Kit Plate 2, well 19A [more] | BBa_I715045 |
| pSB4T5 | NO | NO | NO | |
| BBa_I51020 | YES Kit Plate 1, well 1A |
YES Kit Plate 1, well 3A |
NO | |
| BBa_I716101 | YES [?] Kit Plate 1, well 1C |
NO | NO | |
| BBa_J63009 | YES Kit Plate 1, well 1E |
YES [?] Kit Plate 1, well 3E |
Kit Plate 3, well 2M [more] | BBa_K165018 |
| BBa_J63010 | NO | NO | Kit Plate 3, well 2K [more] | BBa_K165001 |
- If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to make the construction plasmid. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.
We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.
Current Plasmid Backbone Status
Updated on 10/5/09
| Well | Insert | Backbone | Resistance | Current Status | Gel Results | Seq Insert | Seq Plasmid | |
|---|---|---|---|---|---|---|---|---|
| 1A | BBa_J04450 | BBa_I51020 | A | Original 2009 | WRONG | |||
| 1B | BBa_J04450 | BBa_I716101 | A | REASSEMBLED | ||||
| 1C | BBa_J04450 | BBa_J63009 | A | Original 2009 | CONFIRMED | CONFIRMED | ||
| 1D | BBa_J04450 | BBa_J63010 | A | Original 2009 | WRONG | |||
| 1E | BBa_J04450 | pSB1A10 | A | REASSEMBLED | CONFIRMED | |||
| 1F | BBa_J04450 | pSB1A3 | A | REASSEMBLED | CONFIRMED | |||
| 1G | BBa_J04450 | pSB4A5 | A | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 1H | BBa_J04450 | pSB6A1 | A | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 2A | BBa_P1010 | BBa_I51020 | A | Original 2009 | WRONG | |||
| 2B | BBa_P1010 | BBa_I716101 | A | REASSEMBLED | ||||
| 2C | BBa_P1010 | BBa_J63009 | A | REASSEMBLED | CONFIRMED | |||
| 2D | BBa_P1010 | BBa_J63010 | A | REASSEMBLED | ||||
| 2E | BBa_P1010 | pSB1A10 | A | REASSEMBLED | CONFIRMED | |||
| 2F | BBa_P1010 | pSB1A3 | A | Original 2009 | CONFIRMED | CONFIRMED | ||
| 2G | BBa_I52001 | pSB4A5 | A | Original 2009 | CONFIRMED | |||
| 2H | BBa_P1010 | pSB6A1 | A | REASSEMBLED | CONFIRMED | |||
| 3A | BBa_J04450 | pSB1C3 | C | Original 2009 | CONFIRMED | |||
| 3B | BBa_J04450 | pSB3C5 | C | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 3C | BBa_P1010 | pSB1C3 | C | Original 2009 | CONFIRMED | CONFIRMED | ||
| 3D | BBa_I52001 | pSB3C5 | C | Original 2009 | CONFIRMED | |||
| 3E | BBa_I52002 | pSB4C5 | C | Original 2009 | CONFIRMED | |||
| NA | BBa_J04450 | pSB4C5 | C | REASSEMBLED | CONFIRMED | |||
| 4A | BBa_J04450 | pSB1K3 | K | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 4B | BBa_J04450 | pSB2K3 | K | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 4C | BBa_J04450 | pSB3K3 | K | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 4D | BBa_J04450 | pSB4K5 | K | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 4E | BBa_P1010 | pSB1K3 | K | REASSEMBLED | CONFIRMED | |||
| 4F | BBa_P1010 | pSB2K3 | K | REASSEMBLED | ||||
| 4G | BBa_P1010 | pSB3K3 | K | REASSEMBLED | ||||
| 4H | BBa_P1010 | pSB4K5 | K | REASSEMBLED | CONFIRMED | |||
| 5A | BBa_J04450 | pSB1T3 | T | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 5B | BBa_J04450 | pSB3T5 | T | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 5C | BBa_P1010 | pSB1T3 | T | REASSEMBLED | ||||
| 5D | BBa_I52001 | pSB3T5 | T | REASSEMBLED | CONFIRMED | |||
| 5E | BBa_I52001 | pSB4T5 | T | in progress | ||||
| NA | BBa_J04450 | pSB4T5 | T | in progress | ||||
| 6A | BBa_J04450 | pSB1AC3 | AC | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 6B | BBa_P1010 | pSB1AC3 | AC | REASSEMBLED | CONFIRMED | |||
| 7A | BBa_J04450 | pSB1AK3 | AK | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 7B | BBa_J04450 | pSB1AK8 | AK | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 7C | BBa_P1010 | pSB1AK3 | AK | REASSEMBLED | CONFIRMED | CONFIRMED | ||
| 7D | BBa_P1010 | pSB1AK8 | AK | REASSEMBLED | ||||
| 8A | BBa_J04450 | pSB1AT3 | AT | Original 2009 | CONFIRMED | CONFIRMED | ||
| 8B | BBa_P1010 | pSB1AT3 | AT | REASSEMBLED | CONFIRMED | CONFIRMED |