Coding

Part:BBa_K3750000

Designed by: Ying Fei Liew   Group: iGEM21_UNSW_Australia   (2021-10-17)
Revision as of 06:12, 21 October 2021 by Yingfei (Talk | contribs)


GSHR1 glutathione reductase (from Chlamydomonas reinhardtii)

Glutathione reductase (GSR) is a homodimeric flavoprotein (50-kDa subunits). When Glutathione (GSH) neutralises ROS, GSH becomes a radical in the form of oxidised glutathione disulfide (GSSG). GSR regulates cellular glutathione homeostasis by using NADPH and FAD to catalyze the reduction of oxidised glutathione disulfide (GSSG) into glutathione. GSR is highly conserved in aerobic organisms as it is important for cell survival when the organism is subjected to oxidative stress (Carlberg, 1985). In C. reinhardtii, GSR is present in all cellular compartments. This part documents the cytosolic isoform of GSR in C. reinhardtii which is encoded by the gene GSHR1 (Merchant et al., 2007).

GSHR1 catalysis pathway

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 556
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 556
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None