Coding

Part:BBa_K3722004

Designed by: Ruozhou Wang   Group: iGEM21_NWU-CHINA-A   (2021-09-28)
Revision as of 05:18, 21 October 2021 by Liyaa (Talk | contribs) (→‎Biology)


A linker sequence in fusion protein Fre-Linker-SttH

A linker sequence belongs to our fusion protein Fre-Linker-SttH, coding a rigid linker between Fre and SttH.


Biology

After linked the Fre to SttH, the total expression of SttH decreased, but all the chimeric enzymes showed highly increased ratios of their soluble forms in E. coli. The molar concentrations of soluble Fre-Linker–SttH was 1.64 μM, which was more than three times higher than that of SttH by itself[1].

Usage

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None