Tag
FLAG

Part:BBa_M10019

Designed by: John Wang   Group: Berkeley BioE140L - S09   (2009-05-09)
Revision as of 04:17, 10 May 2009 by Johnwang (Talk | contribs)

{˂FLAG˃} Flag is a fusion protein tag. Flag is a short hydrophilic 8-amino acid peptide that binds to the antibody M1. The flag-tag can be at either the N or C terminus of the protein of interest. When purifying, the purification condition of the system is non-denaturing which means it allows for active fusion proteins to be purified. PMID: 12536251

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:


[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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