Part:BBa_K4034004

This part encodes the Thymidylate synthase enzyme.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 64
  Illegal AgeI site found at 70
  Illegal AgeI site found at 604
  
• 1000
  COMPATIBLE WITH RFC[1000]

This part encodes the Thymidylate synthase (TS). It is codon optimised for E. coli BL21.

Description

Thymidylate synthase (TS), has the ability to bind with cellular RNA, forming a complex, which results in translational repression. In general, TS plays an important role in the regulation of the cell cycle, translation, chemosensitivity and apoptosis. Lastly, TS catalyzes the reduction of deoxyuridylate (dUMP) to thymidylate (dTMP), the only de novo method of dTMP production.

Protein Structure

A three secondary stem - loop structure is observed, and this exact structure is hypothesized to influence the TS mRNA translation. With deletion of any one of these repeated sequences, the translational TS mRNA efficiency was altered. The secondary structure of TSase is also important for protein recognition.

Source of this part

NCBI code: AAC75866.1

References

Liu J, Schmitz JC, Lin X, Tai N, Yan W, Farrell M, Bailly M, Chen Tm, Chu E. Thymidylate synthase as a translational regulator of cellular gene expression. Biochim Biophys Acta. 2002 Jul 18;1587(2-3):174-82. doi: 10.1016/s0925-4439(02)00080-7. PMID: 12084459.