Regulatory

Part:BBa_K3930023

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 13:48, 15 October 2021 by Ambre-Jousselin (Talk | contribs)


Galactose inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

The promoter Gal1 is a galactose inducible promoter. This sequence comes from Sacharomyces cerevisiae genome. This part is related to the extensively used and characterized part BBa_J63006. Nonetheless, our promoter lack a Kozak consensus sequence.

Results

Production of α-ionone (BBa_K3930003)

The part (BBa_K3930003) was linearized and transformed into the Sacharomyces.cerevisiae LycoYeast strain. The production of α-ionone was induced by plating the transformants onto YPD media containing 20mg.ml-1 of galactose. Figure 1 shows the GC-MS mass spectra between lysates from induced and non-induced strain lysate.



Figure 1: Production of α-ionone upon galactose induction

α-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak


This pGAL1 promoter without Kozak sequence is functional under these lab conditions.




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