Part:BBa_K3930008

Gene coding for the Linalool synthase

This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome, and has been codon optimized for expression into S.cerevisiae.

Introduction

This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al. 2016).

There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but ours is codon optimized for translation in S. cerevisiae.

References

1. Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 1000
  COMPATIBLE WITH RFC[1000]