Part:BBa_K3852004

T2R4

TAS2R46 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway.

After being stimulated by bitter substances, the bitter receptors activate -gustducin and effector enzyme PDE, and reduce the concentration of intracellular second messenger called ring nucleoside acid(cNMP). After that, calcium channels in the cell membrane were opened to make extracellular calcium ions flow in, and the concentration of intracellular calcium ions increased. Finally, the membrane depolarizes, releasing neurotransmitters.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae.

Usage and Biology

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 (By similarity).

In airway epithelial cells, binding of bitter compounds increases the intracellular calcium ion concentration and stimulates ciliary beat frequency (By similarity).We acquire it from synthesis company.

Reference

[1] Lindemann B. Receptors and transduction in taste[J]Nature,2001,413(6852):219-225.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 278
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]