Part:BBa_K3852003

PHO3

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception.

Several papers have shown that bitter substances are recognized by the bitter receptor TAS2R, a family of G protein-coupled receptors expressed in taste tissue. In humans, more than 25 TAS2Rs have been identified; some of these are activated by bitter substances and function as bitter taste receptors. Because many bitter tastants activate hTAS2R, it has been thought that humans perceive bitterness via those receptors. Experiments show that a variety of bitter peptides can activate T2R4.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae.We acquire it from synthesis company.

Usage and Biology

Gustducin-coupled receptor for denatonium and N6-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. In airway epithelial cells, binding of denatonium increases the intracellular calcium ion concentration and stimulates ciliary beat frequency.

Reference

[1] Mueller, K., Hoon, M., Erlenbach, I. et al. Erratum: The receptors and coding logic for bitter taste. [J]Nature 446, 342 (2007).

[2] Kenji Maehashi, Mami Matano, Hong Wang et al.Bitter peptides activate hTAS2Rs, the human bitter receptors[J]Biochemical and Biophysical Research Communications.2008,365(4): 851-855.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 610
  
• 1000
  COMPATIBLE WITH RFC[1000]