Composite
Part:BBa_K3722010
Designed by: Yongzhu Li Group: iGEM21_NWU-CHINA-A (2021-09-30)
T7_Promoter-RBS-Fre-Linker-SttH-T7_Terminator
Fre is a unique N-terminal tag that efficiently enhanced SttH activity by increasing solubility as well as cofactor supply, the improvement might be caused by an enhanced electron transfer efficiency due to the prox-imity effect of the fusion of Fre and SttH, and greatly improved the yield and productivity of bromination.
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