Composite

Part:BBa_K3475003

Designed by: LI SIYUAN   Group: iGEM20_Tongji_China   (2020-10-21)
Revision as of 23:54, 27 October 2020 by Night12138 (Talk | contribs)


Prm RBS HrtR Ter

Part of heme biosensor system. HrtO repressor, stop repressing when heme exists


Usage and Biology

T--Tongji China--202 2.jpg

FIGURE 2. The relative fluorescence of HBS (E.coli-pHBS-GFP) and WT (E.coli-wildtype).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium with corresponding hemin concentration as blank.

T--Tongji China--202 t.jpg

FIGURE 3. From the result, we can know that the relative fluorescence and and hemin concentration have a linear relation from 0 to 5 μM. It has an obvious difference to wildtype when the hemin concentration is higher than 2 μM.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None