Part:BBa_K3440001

mCherry under bphR1 promoter

PbphR1(BBa_K1155001)-RBS(BBa_B0034)-mCherry(BBa_J06504)-DT(BBa_B0015)

Usage and Biology

Produces mCherry under bphR1 promoter, which can be activated by bphR2:Polychlorinated biphenyl.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Western Blot for BBa_K34440001 (C) and BBa_K3440002 (B)

Fluorescence measurements for BBa_K34440001 (C) and BBa_K3440002 (B)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 156
  Illegal BamHI site found at 239
  Illegal XhoI site found at 46
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 139