Composite

Part:BBa_K3440003

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 22:45, 24 October 2020 by JuCo (Talk | contribs) (→‎Characterization)


GFP under Plux

Plux (BBa_R0062)-RBS(BBa_B0034)-GFP(BBa_E0040)

Usage and Biology

Produces GFP under Plux. Plux can be activated by LuxR:3OC6-HSL.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Colony PCR Gel for BBa_K34440001 (C) and BBa_K3440003 (D)
Fluorescence measurements for BBa_K34440003 (D)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
Parameters
colorgreen
emission530
excitation483
originGFP from Aequorea victoria (Jellyfish) and Plux from Virbio Fischeri