Composite

Part:BBa_K3440008

Designed by: Julie Cordier, Aditi Kallai   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 16:01, 24 October 2020 by JuCo (Talk | contribs) (→‎Usage and Biology)


RhlR with myc tag under constitutive promoter


Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-Myc(BBa_K823036)-DT(BBa_B0015)


Usage and Biology

Produces RhlR-Myc under constitutive promoter. RhlR can be complexed with C4-HSL and activate Prhl.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/signalling/receiver
Parameters
origin Pseudomonas aeruginosa
proteinRhlR
tagmyc