Composite

Part:BBa_K3365061:Design

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-23)
Revision as of 12:24, 26 October 2020 by Gyaxxxxxx (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Target transcription activating unit upstream of RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 66
    Illegal NheI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 812
    Illegal XhoI site found at 803
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 667
    Illegal AgeI site found at 779
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We constructe this part by PCR and then add homologous region to the fragment by performing another PCR for assemble fragments onto plasmids by Gibson Assembly.

References