Part:BBa_K3447007
xynD, which produces xylanase
T7 polymerase stage 2 with pMag.
Usage and Biology
In our project, we divided T7 RNA polymerase into two parts, respectively called T7NT (BBa_K3447008) and T7CT (BBa_K3447007) and attached on the nMag and pMag. Under blue light irradiation, these two parts undergo specific blue light dependent heterodimerization and assemble into complete T7 RNA polymerase, which acts on PT7 promoter (BBa_K3447005) and activates downstream gene expression. And the process is reversible, that is, when blue light is stopped, the dimerized T7 RNA polymerase dedimers and loses its function.
In our project, we use this part to form a complete T7 RNA polymerase by dimerizing it with T7NT under blue light. (链接104+f09+104+f10+001+b17)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 216
Illegal PstI site found at 226 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 216
Illegal PstI site found at 226 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 216
Illegal PstI site found at 226 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 216
Illegal PstI site found at 226 - 1000COMPATIBLE WITH RFC[1000]
Design
Design Notes
We added some synonymous mutations to avoid part rules.
- Armin Baumschlager, Stephanie K. Aoki, and Mustafa Khammash, Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control, ACS Synth. Biol. 2017, 6, 2157-2167.
References
1. Armin Baumschlager, Stephanie K. Aoki, and Mustafa Khammash, Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control, ACS Synth. Biol. 2017, 6, 2157-2167.
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