Device
Part:BBa_K3629016
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-18)
Modified T. reesei EGI expression construct with gibson homology sequences and 6X His tag
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2385
Illegal EcoRI site found at 2204 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2204
Illegal NheI site found at 81
Illegal SpeI site found at 2386
Illegal PstI site found at 2400
Illegal NotI site found at 7
Illegal NotI site found at 2393 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2204
Illegal BamHI site found at 2334
Illegal XhoI site found at 132 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2386
Illegal EcoRI site found at 2204 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2204
Illegal XbaI site found at 16
Illegal SpeI site found at 2386
Illegal PstI site found at 2400
Illegal NgoMIV site found at 1326 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |