Regulatory
Part:BBa_K3408000
Designed by: Fengzhi Yuan Group: iGEM20_NAU-CHINA (2020-10-02)
Revision as of 13:55, 5 October 2020 by NJAU-Chappie (Talk | contribs)
We add the promoter “Pnarnar”, in response to the RES-FNR oxygen regulator system of Bacillus Subtilis, to express phy(ycD) phytase efficiently in the absence of oxygen, and meanwhile express CI repressor protein to inhibit toehold switch-Dpnc downstream.
According to the paper, they have designed three kinds of strength of the promoter Pnarnar and applied them in E.coli successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.
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