Regulatory

Part:BBa_K3408000:Design

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-02)
Revision as of 01:06, 28 October 2020 by NJAU-Chappie (Talk | contribs) (→‎Design Notes)


promoter nar(fnr regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized.

Source

The sequence is found through the paper <Engineering and application of synthetic nar promoter for fne-tuning the expression of metabolic pathway genes in Escherichia coli>.

References

Wang Xi, Lu Wenliang, Yao Mingze, et al. Heterologous expression and purification of the phytase phy (ycD) gene from Bacillus subtilis in Escherichia coli[J]. Applied and Environmental Biology, 2014, 20(02):295-299.