Template:TypeIIS/Plasmids/Level1

Revision as of 20:15, 17 June 2020 by Vinoo (Talk | contribs)

Level 1 transcriptional units in this set of plasmid backbones will be flanked by SapI recognition sites and fusion sites, enabling further assembly of up to 4 transcriptional units as a Level 2 Assembly.

Registry Name Loop Name Fusion Site 5' TU Fusion Site 3'
pSB1K01 pOdd1 ATG TU 1 GCA
pSB1K02 pOdd2 GCA TU 2 TAC
pSB1K03 pOdd3 TAC TU 3 CAG
pSB1K04 pOdd4 CAG TU 4 GGT


TypeIIS-Level1-J04454-Simple.png
Simplified diagram of BBa_J04454 and pSB1K0# with BsaI cut site.


When four Level 0 parts are assembled into the following plasmid backbones (with BBa_J04454), the resulting transcriptional unit (TU) will be flanked by SapI restriction sites and these 3 bp Fusion Sites (NNN), which can be used for subsequent (Level 2) assemblies.

TypeIIS-Level1-L0-Part-Assembly-Simple.png
TypeIIS-Level1-TU-Simple.png



Note:

  • Since pSB1K3 is a high-copy plasmid backbone, these vectors should not be used for Level 3 assemblies.
  • Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04454 have been verified.