Template:TypeIIS/Plasmids/Level1

Revision as of 19:51, 15 June 2020 by Vinoo (Talk | contribs)

When four Level 0 parts are assembled into a Transcriptional Unit (TU) into the following plasmid backbones (with BBa_J04454), the TU will be flanked by SapI restriction sites and these 3 bp Fusion Sites.

Registry Name Loop Name Fusion Site 5' TU Fusion Site 3'
pSB1K01 pOdd1 ATG TU 1 GCA
pSB1K02 pOdd2 GCA TU 2 TAC
pSB1K03 pOdd3 TAC TU 3 CAG
pSB1K04 pOdd4 CAG TU 4 GGT
Note: Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert BBa_J04454 have been verified.