Coding

Part:BBa_K3078003:Design

Designed by: Ziang Guo   Group: iGEM19_Jilin_China   (2019-10-14)
Revision as of 00:10, 22 October 2019 by Nashor (Talk | contribs) (→‎References)

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Bcam0581


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 26
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Frame shift mutation should be avoided.

Source

The sequence was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/NC_011001.1?report=genbank&from=645965&to=646828&strand=true


References

[1]Boon C, Deng Y, Wang L H,et al. A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition[J]. ISME J, 2008, 2(1): 27-36.

[2]Bi H, Christensen Q H, Feng Y, et al. The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities[J]. Mol Microbiol, 2012, 83(4): 840-55.