Terminator

Part:BBa_K3046018:Design

Designed by: Philip Srensen, Marcus Medom Ryding   Group: iGEM19_DTU-Denmark   (2019-10-16)
Revision as of 00:07, 22 October 2019 by Lookingforanswer (Talk | contribs) (→‎Source)

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Ttef (Domesticated from Aspergillus nidulans)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 138
    Illegal BamHI site found at 398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence for this promoter was identified from the Aspergillus nidulans genome, and a single basepair was changed from the wildtype version in order to domesticate the terminator.

Source

Based on native TEF-terminator from A. nidulans

References