DNA
Part:BBa_K2935070:Design
Designed by: Haiyong Shu Group: iGEM19_FAFU-CHINA (2019-10-16)
a promoter of ChR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1560
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1560
Illegal NheI site found at 351
Illegal NheI site found at 414 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1560
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1560
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1560
Illegal NgoMIV site found at 764
Illegal AgeI site found at 670 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1658
Design Notes
The promoter in front of ChR gene as the photosensitive promoter. It can be activated in blue light to promote expression.
Source
Chlamydomonas reinhardtii