Part:BBa_K3053042

Beta lactamase mRNA targeting sequence

This part is the culmination of all that we have done. This will produce the antisense mRNA which is designed to target the beta lactamase gene in pBR322 which confers antibiotic resistance against the antibiotic ampicillin.

Usage and Biology

This part works when there is the beta lactamase gene in the organism it is transformed with. Under this condition, the bla (shortened beta lactamase) mRNA is targeted by the mRNA produced by our part. This forms a double stranded mRNA covering the RBS region of the bla which causes the bla mRNA to degrade.

As a result of this, no beta lactamase is produced removing the bacteria's resistance to ampicillin. In the absence of a bla gene the sense part of our construct BBa_K3053069 (https://parts.igem.org/Part:BBa_K3053069) forms a self complementary stem loop and ensures its further degradation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 204
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]