Plasmid

Part:BBa_K2908670

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-16)
Revision as of 14:19, 21 October 2019 by Lucas-lzj (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pLN431-G8p-yCD-miR101BS

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of yCD and miR101-BS for sequence-specific expression of yCD .Therefore, we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 so that regulate the transactivation domain of yCD(BBa_K2908667).CD converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death. T--CSU_CHINA--2908670.jpg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 197
    Illegal NheI site found at 1143
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 244
    Illegal XhoI site found at 60
    Illegal XhoI site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 755
    Illegal BsaI.rc site found at 792


[edit]
Categories
Parameters
None