Protein_Domain

Part:BBa_K2908667:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-16)
Revision as of 21:12, 17 October 2019 by Sqx98625 (Talk | contribs) (→‎Design Notes)

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yCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 854
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 466
    Illegal BsaI.rc site found at 503


Design Notes

We must use the sequence of it correctly or yCD cannot work effectively. T--CSU_CHINA--yCD1.png

Source

We get the sequence from NCBI and synthesized it by company.

References

Ko, T.-P., Lin, J.-J., Hu, C.-Y., Hsu, Y.-H., Wang, A.H.-J., Liaw, S.-H, Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution, (2003) J.Biol.Chem. 278: 19111-19117.