Composite

Part:BBa_K3187012

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 12:10, 17 October 2019 by ToPro (Talk | contribs)


pTet sfGFP-P22 Bacteriophage Scaffolding Protein x Coat Protein pT7 (Convergent)

  • Name: pTeTW3con2-ptet-sfGFP-StrepTag-SP—CP+LPETGG-pT7
  • Size [bp]: 2932
  • Size [kDa]: 46.1 (sfGFP – Strep-Tag – Scaffold Protein) + 48.9 (Coat Protein - Strep-Tag - LPETGG)
  • Origin: Synthetic?
  • Components: tetA promoter, T7 promoter, T7 terminator, Lac Operator, RBS, Coat Protein, Scaffold Protein, sfGFP, LPETGG, Strep-Tag II
  • Feature: Production of P22 Virus-like particles with a modifiable surface and sfGFP as cargo


z




Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 2900
    Illegal PstI site found at 1334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NheI site found at 1339
    Illegal PstI site found at 1334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal BamHI site found at 796
    Illegal XhoI site found at 1499
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 2900
    Illegal PstI site found at 1334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal XbaI site found at 2900
    Illegal PstI site found at 1334
    Illegal NgoMIV site found at 1185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 94


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Parameters
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