Part:BBa_K2984027

L1c-Psad-ARS-YFP-Rbcs2

L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1110
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1110
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1110
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1110
  Illegal NgoMIV site found at 1587
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

<figure> <img src="igem" alt="colonies_total" width="500"> <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> </figure>