Part:BBa_E0022

enhanced cyan fluorescent protein derived from A. victoria GFP

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

In ECUST_China 2019 characterization，we constructed a plasmid containing pLac and CI protein, adding BBa_E0022 eCFP as the reporter gene. We attempted to verify the function of this plasmid via observing the eCFP fluorescence by the comparison between the negative control and induced cells.

Figure 1. Fluorescence intensity of eCFP

However, since the CI and eCFP was polycistron, both under the control of pLac, the expression of eCFP was disturbed by CI. The figure illustrated that as time went by, the expression of eCFP decreased, contrary to the prospective results. The characterization of eCFP in ECUST_China showed that BBa_E0022 was not suitable for the characterization of polycistron structure.